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As these data did not meet the requirements of a parametric analysis of variance (ANOVA), a non-parametric one-way analysis ANOVA was performed to test the hypothesis that there were significant differences between median ice nucleation temperature using a two-tailed KruskalWallis test on ranks

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Measurement of Protein Expression by ELISA The extracellular content and release of IL-1, IL-8, HSPA1A and FOSL1 were determined using ELISA

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